Flow Cytometry Lab
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The Flow Cytometry Laboratory
Room 311D
Department of Pathology
The University of Texas
Health Science Center at San Antonio
San Antonio, Texas
78284-7750
Phone: (210) 567-4096
Fax: (210) 567-2367



The Flow Cytometry Laboratory provides complete immunophenotypic analysis by multi parameter flow cytometry and detection of terminal deoxynucleotidase (TdT) by immunofluorescence staining. The assays are of value in the diagnosis and surveillance of Leukemia (acute and chronic), Lymphoma, and immunodeficiency and immune altered states.

Leukemia and lymphoma

Flow cytomoetry and TdT staining are useful in the diagnosis and classification of acute leukemia, the diagnosis and classification of non-Hodgkin's lymphoma and the detection of residual leukemia or lymphoma following therapy. Analysis by the Flow Cytometry Laboratory includes morphologic evaluation by a pathologist with experience in hematopathology, selection of a suitable panel of immunophenotypic markers, notification of results by telephone within 24 hours of receipt of the specimen and mailed or faxed report including an interpretation of the data.


Immunodeficiency states

Flow cytometry is used for the monitoring of Human Immunodeficiency Virus (HIV) infection, diagnosis of congenital immunodeficiency states, and analysis of iatrogenic immunodeficiency. The Flow Cytometry Laboratory performs a complete immunophenotypic analysis following guidelines provided by the CDC. The report includes the perecentages and absolute counts for T-cell subsets, B-cell and natural killer (NK) cells, as well as a summary of any previous results.


Immune altered states

Flow cytometry is of value in detecting and monitoring immune altered states associated with autoimmune diseases, exposure to toxic chemicals, and drug therapy.


Specimen Requirements:

Sample Requirements Storage / Shipping
Peripheral blood 5-10 ml in EDTA room temperature, less than 24 hours old
Bone marrow 1-2 ml in EDTA room temperature, less than 24 hours old
Pleural fluid at least 500 ml refrigerate
Ascitic fluid at least 500 ml refrigerate
Lymph node minced in RPMI refrigerate
Fine needle aspirate suspend in RPMI refrigerate


For CSF and other specimens not listed above, call the laboratory for handling instructions.

Please notify the laboratory before sending a specimen.

 

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